导师介绍
陈扬 副研究员
陈扬
副研究员，博士生导师
北京大学临床医学高等研究院精准医疗多组学研究中心
北京大学医学部bat365官网登录入口生物化学与分子生物学系
研究方向：细胞外囊泡/蛋白质组学
办公地址：昌平医疗产业园12号楼1层
Email: chenyang1816185048@bjmu.edu.cn
研究领域及方向：
主要研究方向为膜性细胞器发生和基于质谱到蛋白组学的研究。研究外泌体、新细胞器迁移体等细胞外囊泡发生、物质分选运输、细胞间信号沟通的分子细胞生物学机制。应用蛋白质谱技术大规模地发掘基于疾病问题和临床队列的生物标志物，探索疾病发生规律，重点关注基于细胞外囊泡的疾病诊断标志物鉴定和疾病发生发展机制。
简历：
2024-至今 副研究员（Tenure track）：北京大学临床医学高等研究院精准医疗多组学中心／bat365官网登录入口
2018—2023 副研究员（Tenure track）：精准医疗多组学中心／bat365官网登录入口
2016—2018 副研究员：清华大学生命科学学院
2014—2016 助理研究员：清华大学生命科学学院
2012—2014 博士后：清华大学生命科学学院（合作导师：俞立）
2008—2012 博士：北京大学医学部基础医学专业（美国国立卫生研究院）
（导师：柯杨、Matthias Machner）
2004—2008 本科：北京大学医学部基础医学专业
基金：
国家重点研发专项（2018-2023，课题骨干，250万元），
香港大学、伦敦帝国理工学院、北京大学联合建立合成化学与化学生物学实验室（2020-2025，课题骨干，100万元），
科技部脑计划青年项目（2022-2026，参与，100万元），
国家重点研发计划重点专项（2023-2026，课题骨干，70万元），
国家自然科学基金委青年项目（2024-2026，主持，30万元）
论文：
研究性论文：“*”代表第一作者；“#”代表责任作者
1.Yong Li*, Yiling Wen*, Ying Li, Xinyi Tan, Shuaixin Gao, Peiyao Fan, Wenmin Tian, Catherine CL Wong#, Yang Chen#. Rab10-CAV1 mediated intraluminal vesicles transport to migrasomes. PNAS. in press.
2.Wenmin Tian*, DongXue Shi*, Yinmei Zhang*, Hongli Wang, Haohao Tang, Zhongyu Han, Catherine CL Wong, Liyan Cui#, Jiajia Zheng#, Yang Chen#. Deep proteomic analysis of obstetric antiphospholipid syndrome by DIA-MS of large and small circulating extracellular vesicle enriched fraction. Communications Biology. 2024 Jan 15;7(1):99.
3.Yinmei Zhang*, Shangjia Jin*, Wenmin Tian*, Dongxue Shi, Yang Chen#, Liyan Cui#, Jiajia Zheng#. Proteomic of serum samples for the exploration of the pathological mechanism of Obstetric Antiphospholipid Syndrome. Journal of Proteome Research. 2024 Jan 5;23(1):289-300. Selected Cover.
4.Yang Chen*, Nan Zhang*, Jie Zhang*, Jiangtao Guo*, Shaobo Dong*, Heqiang Sun, Shuaixin Gao, Tingting Zhou, Min Li, Xueyuan Liu, Yaxin Guo, Beiwei Ye, Yingze Zhao, Tongqi Yu, Jianbo Zhan, Yongzhong Jiang, Catherine CL Wong#, George F. Gao#, William J. Liu#. Immune response pattern across the asymptomatic, symptomatic and convalescent periods of COVID-19. Biochimica et Biophhysica Acta proteins and proteomics. 2022 Feb 1; 1870(2): 140736. Epub ahead of print. (Cover story)
5.Wenmin Tian*, Delin Li*, Nan Zhang*, Guijie Bai, Kai Yuan, Haixia Xiao, Feng Gao, Yang Chen#, Catherine C L Wong#, George Fu Gao#. O-glycosylation pattern of the SARS-CoV-2 spike protein reveals an "O-Follow-N" rule. Cell Research. 2021 Oct;31(10):1123-1125.
6.Yang Chen*, Hangping Yao*, Nan Zhang*, Jie Wu*, Shuaixin Gao, Jiangtao Guo, Xiangyun Lu, Linfang Cheng, Rui Luo, Xue Liang, Catherine CL Wong#, Min Zheng#. Proteomic analysis identifies prolonged disturbances in pathways related to cholesterol metabolism and myocardium function in the COVID-19 recovery stage. Journal of Proteome Research. 2021 Jul 2;20(7):3463-3474
7.Wenmin Tian*, Nan Zhang*, Ronghua Jin*, Yingmei Feng*, Siyuan Wang*, Shuaixin Gao, Ruqin Gao, Guizhen Wu, Wenjie Tan*, Yang Chen#, George, Fu Gao#, and Catherine CL Wong#(corresponding author). Immune suppression in the early stage of COVID-19 disease. Nature Communications. 2020, 11(1):5859.
8.Puzhong Lu, Rui Liu, Di Lu, Yue Xu, Xueyi Yang, Zheng Jiang, Chun Yang, Li Yu#, Xiaoguang Lei#, Yang Chen# (2020). Chemical screening identifies ROCK1 as a regulator of migrasome formation. Cell Discovery, 2020 Aug 4;6:51.
9.Xiaoxin Zhao*, Yaxin Lei, Jiajia Zheng, Junya Peng, Ying Li, Li Yu#, and Yang Chen# (2019). Identification of markers for migrasome detection. Cell Discovery, 2019 May 21;5:27. (co-corresponding author)
10.Na Mi*, Yang Chen*, Shuai Wang, Mengran Chen, Mingkun Zhao, Guang Yang, Meisheng Ma, Qian Su, Sai Luo, Jingwen Shi, Jia Xu, Qiang Guo, Ning Gao, Yujie Sun, Zhucheng Chen, Li Yu. CapZ regulates autophagosomal membrane shaping by promoting actin assembly inside the isolation membrane. Nature Cell Biology. 2015 Jan; 25(1): 24-38 (co-first author)
11.Wanqing Du*, Qian Peter Su*, Yang Chen*, Yueyao Zhu, Dong Jiang, Yueguang Rong, Senyan Zhang, Yixiao Zhang, He Ren, Chuanmao Zhang, Xinquan Wang, Ning Gao, Yanfeng Wang, Lingfei Sun, Yujie Sun, Li Yu. Kinesin 1 Drives Autolysosome Tubulation. Developmental Cell. 2016 May 23; 37(4): 326-36 (co-first author)
12.M Ramona Neunuebel*, Yang Chen*, Andrew H. Gaspar, Peter S. Backlund Jr, Alfred Yergey, Matthias P. Machner. De-AMPylation of the small GTPase Rab1 by the pathogen Legionella pneumophila. Science. 2011 Jul 22; 333(6041): 453-6 (co-first author)
13.Yang Chen*, Igor Tascón*, M Ramona Neunuebel, Chiara Pallara, Jacqueline Brady, Lisa N. Kinch, Juan Fernández-Recio, Adriana L. Rojas, Matthias P. Machner, Aitor Hierro. Structural basis for Rab1 de-AMPylation by the Legionella pneumophila effector SidD. PLOS Pathogen. 2013 May 16; (co-first author)
14.Yang Chen*, Matthias P. Machner. Targeting of the small GTPase Rab6A' by the Legionella pneumophila effector LidA. Infection and Immunity. 2013 Jun 881(6): 2226-35
综述和评论性文章：
1.Chen Y*, Su Q*, Sun YJ, Yu L. Visualizing autophagic lysosome reformation in cells using in vitro reconstitution systems. Current Protocols in Cell Biology, 2018 Mar;78(1):11.24.1-11.24.15.
2.Chen Y*, Li Y, Ma L, Yu L. Detection of migrasomes. Cell Migration: Methods and Protocols, Methods in Molecular Biology, vol. 1749, 2018
3.Chen Y*, Li Y. Development of research into autophagic lysosome reformation. Molecules and Cells. 2018 Jan 31;41(1):45-49.
4.Yu L, Chen Y, Tooze SA. Autophagy pathway: cellular and molecular mechanisms. Autophagy. 2017 Sep 21:1-9.
5.Chen Y*, Yu L, Recent progress in autophagic lysosome reformation. Traffic. 2017 Jun; 18(6): 358-361.
6.Chen Y*, Yu L, Research progress of membranous organelles and their subcellular structures in China. Chinese Science Bulletin 2017 May 12;
7.Chen Y*, Yu L, Scissors for autolysosome tubules. EMBO J. 2015 Sep 2; 34(17): 2217-8.
8.Chen Y*, Yu L. Autophagic lysosome reformation. Exp Cell Res. 2013 Jan 15;319(2):142-6
9.Machner MP*, Chen Y, Catch and release: Rab1 exploitation by Legionella pneumophila. Cell Logist. 2011 Jul 1; 1(4): 133-138.